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Saturday, December 31, 2011
Assay of Ascorbic acid
Assay of Ascorbic acid
Reagents Required:
1.0M Sulfuric acid
0.05M Iodine
Procedure
Weigh accurately about 0.1gm of sample and dissolve in a mixture of 100 ml freshly boiled and cooled water and 25ml of 1M sulfuric acid. Immediately Titrate with 0.05M iodine, using starch solution as indicator until a persistent blue-violet color is obtained. Each ml of 0.05M iodine is equivalent to 0.008806gm of ascorbic acid.
Calculation:
BR X Actual molarity X 0.008806 X 100
% of Ascorbic acid = ----------------------------------------------------
0.05 X Wt. Of sample
BR= Burette Reading
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